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- Title
Transcriptional profiling upon T cell stimulation reveals down-regulation of inflammatory pathways in T and B cells in SLE versus Sjögren's syndrome.
- Authors
Kwon, Gino; Wiedemann, Annika; Steinheuer, Lisa M.; Stefanski, Ana-Luisa; Szelinski, Franziska; Racek, Tomas; Frei, Andreas Philipp; Hatje, Klas; Kam-Thong, Tony; Schubert, David; Schindler, Thomas; Dörner, Thomas; Thurley, Kevin
- Abstract
Systemic lupus erythematosus (SLE) and primary Sjögren's syndrome (pSS) share clinical as well as pathogenic similarities. Although previous studies suggest various abnormalities in different immune cell compartments, dedicated cell-type specific transcriptomic signatures are often masked by patient heterogeneity. Here, we performed transcriptional profiling of isolated CD4, CD8, CD16 and CD19 lymphocytes from pSS and SLE patients upon T cell stimulation, in addition to a steady-state condition directly after blood drawing, in total comprising 581 sequencing samples. T cell stimulation, which induced a pronounced inflammatory response in all four cell types, gave rise to substantial re-modulation of lymphocyte subsets in the two autoimmune diseases compared to healthy controls, far exceeding the transcriptomic differences detected at steady-state. In particular, we detected cell-type and disease-specific down-regulation of a range of pro-inflammatory cytokine and chemokine pathways. Such differences between SLE and pSS patients are instrumental for selective immune targeting by future therapies.
- Subjects
B cells; SJOGREN'S syndrome; T cells; SYSTEMIC lupus erythematosus; LYMPHOCYTE subsets; AUTOIMMUNE diseases; CHEMOKINE receptors; T cell receptors
- Publication
NPJ Systems Biology & Applications, 2023, Vol 9, Issue 1, p1
- ISSN
2056-7189
- Publication type
Article
- DOI
10.1038/s41540-023-00319-z