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- Title
Biological Effects of New Hydraulic Materials on Human Periodontal Ligament Stem Cells.
- Authors
López-García, Sergio; Lozano, Adrián; García-Bernal, David; Forner, Leopoldo; Llena, Carmen; Guerrero-Gironés, Julia; Moraleda, José M.; Murcia, Laura; Rodríguez-Lozano, Francisco J.
- Abstract
Background: The aim of this study was: to evaluate the biological properties of new hydraulic materials: Bio-C Repair and Bio-C Sealer. Methods: Periodontal ligament stem cells were exposed to several dilutions of Bio-C Repair and Bio-C Sealer. The ion release profile and pH were determined. Metabolic activity, cell migration and cell survival were assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), wound-healing assays and Annexin assays, respectively. Cells were cultured in direct contact with the surface of each material. These were then analyzed via scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). Statistical differences were assessed using a two-way ANOVA (α < 0.05). Results: Similar pH was observed in these cements. Bio-C Sealer released significantly more Ca and Si ions (p < 0.05) in comparison with Bio-C Repair. Undiluted Bio-C Sealer induced a significant reduction on cellular viability, cell survival and cell migration when compared to the control (p < 0.05). Moreover, SEM showed abundant cells adhered on Bio-C Repair and a moderate number of cells attached on Bio-C Sealer. Finally, EDX analysis identified higher percentages of Ca and O in the case of Bio-C repair than with Bio-C sealer, while other elements such as Zr and Si were more abundant in Bio-C sealer. Conclusions: Bio-C Repair displayed higher cell viability, cell adhesion and migration rates than Bio-C Sealer.
- Subjects
PERIODONTAL ligament; STEM cells; CELL migration; ACCULTURATION; SCANNING electron microscopy
- Publication
Journal of Clinical Medicine, 2019, Vol 8, Issue 8, p1216
- ISSN
2077-0383
- Publication type
Article
- DOI
10.3390/jcm8081216