We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Propofol suppresses invasion and induces apoptosis of osteosarcoma cell in vitro via downregulation of TGF-β1 expression.
- Authors
XU, Y.-B.; JIANG, W.; ZHAO, F.-R.; LI, G.; DU, Q.-H.; ZHANG, M.-Y.; GUO, X.-G.
- Abstract
OBJECTIVE: Osteosarcoma (OS) is the most common malignant tumor of the bone, with a high mortality rate and poor prognosis. Propofol has been proposed to play a role of antitumor in various cancers. However, the functions and mechanisms of propofol in OS is still not clear. MATERIALS AND METHODS: The different concentrations of propofol were co-incubated with osteosarcoma MG-63 lines for 72 hrs. Cell proliferation, apoptosis, and invasion were detected by MTT assay, Flow cytometry analysis, and Matrigel invasion assay. Western blot was used to detect the TGF-β1 protein levels. MG-63 cells were treated with human recombinant TGF-β1 (rh TGF-β1) to assess the role of TGF- β1 in propofol-induced anti-tumor activity. RESULTS: Propofol significantly inhibited cell proliferation and invasion and promoted apoptosis of MG-63 lines cells. Propofol also efficiently reduced TGF-β1 expression. Moreover, restoration of TGF-β1 by rhTGF-β1 treatment reversed the effects of propofol on the biological behavior of OS cells. CONCLUSIONS: Propofol can effectively inhibit proliferation and invasion and induce apoptosis of OS cells through, at least partly, downregulation of TGF-β1 expression.
- Subjects
PROPOFOL; OSTEOSARCOMA; TRANSFORMING growth factors-beta; ANTINEOPLASTIC agents; DOWNREGULATION; FLOW cytometry
- Publication
European Review for Medical & Pharmacological Sciences, 2016, Vol 20, Issue 7, p1430
- ISSN
1128-3602
- Publication type
Article