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- Title
Expression of c-FLIP in malignant melanoma, and its relationship with the clinicopathological features of the disease.
- Authors
Tian, F.; Lu, J. J.; Wang, L.; Li, L.; Yang, J.; Li, Y.; Liu, Y. Q.; Shen, G. X.; Tu, Y. T.; Tao, J.
- Abstract
Summary Background. Numerous molecular events have been associated with the development of malignant melanoma (MM). The cellular FLICE-like inhibitory protein (c-FLIP) was originally identified as an inhibitor of death-receptor signalling through competition with FADD-like interleukin-1β-converting enzyme (FLICE; also known as caspase-8) for recruitment to the FAS-associated death domain (FADD), and it has been suggested recently that c-FLIP is required for the survival and proliferation of various cell types. Aim. To investigate the relationship of c-FLIP expression with the clinicopathological features of MM. Methods. Immunohistochemical staining with anti-c-FLIP antibody was performed on tissue samples taken from 77 MMs and 20 naevi. The proliferative cells were visualized by staining with Ki-67 antibody. Annexin V-propidium iodide labelling, a marker for chromatin condensation, was performed to observe the rate of cell apoptosis by flow cytometry. Results. Expression of c-FLIP was increased in MM tissue compared with the matched pigmented naevi. The c-FLIP expression was significantly associated with the histological type and Clark level of MM, and correlated strongly with the Ki-67 labelling index. Downregulation of c-FLIP might increase apoptosis in MM cell lines. Conclusions. c-FLIP might play an important role in the obtaining of aggressive biological behaviours and be useful in predicting prognosis of patients with MM.
- Subjects
MELANOMA; PROTEINS; INTERLEUKINS; IMMUNOGLOBULINS; CHROMATIN; APOPTOSIS; ENZYME inhibitors; PATIENTS
- Publication
Clinical & Experimental Dermatology, 2012, Vol 37, Issue 3, p259
- ISSN
0307-6938
- Publication type
Article
- DOI
10.1111/j.1365-2230.2011.04238.x