We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Reverse effect of curcumin on CDDP-induced drug-resistance via Keap1/p62-Nrf2 signaling in A549/CDDP cell.
- Authors
Shen, Jie; Chen, Ya-Juan; Jia, Yuan-Wei; Zhao, Wen-Ying; Chen, Guang-Hai; Liu, Ding-Feng; Chen, Yun-Yu; Zhang, Chao; Liu, Xiao-Ping
- Abstract
Objective To assess the effect of curcumin on CDDP-induced drug resistance and explore the underlying molecular mechanism through Nrf2 system and autophagy pathway. Methods A drug-resistant cell model was established by exposing A549/CDDP cell to 2 μg/mL CDDP. A549/CDDP cell was treated with 20 μg/mL CDDP and 10 μM curcumin. The cell viability and apoptosis level, the signals of Keap1/P62-Nrf2 and autophagy pathway were analyzed. Results CDDP induction promoted drug-resistant phenotype in A549/CDDP cell and activated autophagy as well as Nrf2 signals in A549/CDDP cell. Meanwhile, curcumin combination attenuated autophagy and Nrf2 activation induced by CDDP, and reversed the drug-resistant phenotype. Notably, curcumin combination augmented Keap1 transcription. Furthermore, Keap1 ablation with short hairpin RNAs hampered the efficacy of curcumin, suggesting Keap1 played a crucial role on reversal effect of curcumin. Conclusions The present findings demonstrate that CDDP promotes abnormal activation of Nrf2 pathway and autophagy, leading to drug resistance of A549/CDDP cell. Curcumin attenuates this process and combat drug-resistance through its potent activation on Keap1 transcription, which is essential for interplay between oxidative stress induced Nrf2 activation and autophagy/apoptosis switch.
- Publication
Asian Pacific Journal of Tropical Medicine, 2017, Vol 10, Issue 12, p1190
- ISSN
1995-7645
- Publication type
Article
- DOI
10.1016/j.apjtm.2017.10.028