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- Title
Rapid Determination of Ebolavirus Infectivity in Clinical Samples Using a Novel Reporter Cell Line.
- Authors
Kainulainen, Markus H.; Nichol, Stuart T.; Albariño, César G.; Spiropoulou, Christina F.
- Abstract
Modern ebolavirus diagnostics rely primarily on quantitative reverse transcription-polymerase chain reaction (qRT-PCR), a sensitive method to detect viral genetic material in the acute phase of the disease. However, qRT-PCR does not confirm presence of infectious virus, presenting limitations in patient and outbreak management. Attempts to isolate infectious virus rely on in vivo or basic cell culture approaches, which prohibit rapid results and screening. In this study, we present a novel reporter cell line capable of detecting live ebolaviruses. These cells permit sensitive, large-scale screening and titration of infectious virus in experimental and clinical samples, independent of ebolavirus species and variant.
- Subjects
REVERSE transcriptase polymerase chain reaction; VIRAL genetics; EBOLA virus disease; RNA viruses; CYTOPLASM; ANIMALS; CELL culture; CELL lines; GENOMES; MOLECULAR diagnosis; POLYMERASE chain reaction; PRIMATES; RNA; EBOLA virus; EQUIPMENT &; supplies; DIAGNOSIS
- Publication
Journal of Infectious Diseases, 2017, Vol 216, Issue 11, p1380
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jix486