We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Hyperkeratosis in human lower limb lymphedema: the effect of stagnant tissue fluid/lymph.
- Authors
Domaszewska ‐ Szostek, A.; Zaleska, M.; Olszewski, W.L.
- Abstract
Background Hyperkeratosis of skin in lower limb lymphedema is one of the sequelae of tissue fluid/lymph ( TF/L) stasis, but its mechanisms remain unknown. It is noteworthy, nonetheless, that human TF/L contains high levels of growth factors and cytokines, and may serve as the physiological environment for keratinocyte ( KC) proliferation. Objective The aim of the study was to investigate the effect of human TF/L on human KC proliferation, differentiation and on the expression of epidermal stem cell markers on them. Methods KC were isolated from lymphedema and normal skin, and cultured for 1-14 days in TF/L with neutralized Interleukin 1β, Interleukin 6, tumour necrosis factor α ( TNF-α), keratinocyte growth factor ( KGF) or tumour growth factor β ( TGF-β). Alternatively, KC receptors for these factors were blocked. Results The number of KC cultured in TF/L was increased, as was the percentage of mitotic figures. There was a higher percentage of p63, CD29, Ki67, PCNA, CK6, CK17, CK16 and a lower of CK10, CK14, filaggrin and involucrin-positive KC. Neutralization of TF/L IL-1β, IL-6, TNF-α and KGF as well as blockage of their receptors resulted in decreased percentage of mitotic KC. TGF-β had a limited effect on KC proliferation. Conclusion Hyperkeratosis in lymphedema may be the effect of a high concentration of cytokines in the stagnant TF/L tissue, but not because of presumed changes in the KC.
- Subjects
KERATOSIS; LYMPHEDEMA diagnosis; SKIN disease treatment; LEG diseases; KERATINOCYTE growth factors; DIAGNOSIS
- Publication
Journal of the European Academy of Dermatology & Venereology, 2016, Vol 30, Issue 6, p1002
- ISSN
0926-9959
- Publication type
Article
- DOI
10.1111/jdv.13565