We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Integrated miRNA and mRNA expression profiling of the inflammatory breast cancer subtype.
- Authors
Van der Auwera, I.; Limame, R.; van Dam, P.; Vermeulen, P. B.; Dirix, L. Y.; Van Laere, S. J.
- Abstract
<bold>Background: </bold>MicroRNAs (miRNAs) are key regulators of gene expression. In this study, we explored whether altered miRNA expression has a prominent role in defining the inflammatory breast cancer (IBC) phenotype.<bold>Methods: </bold>We used quantitative PCR technology to evaluate the expression of 384 miRNAs in 20 IBC and 50 non-IBC samples. To gain understanding on the biological functions deregulated by aberrant miRNA expression, we looked for direct miRNA targets by performing pair-wise correlation coefficient analysis on expression levels of 10 962 messenger RNAs (mRNAs) and by comparing these results with predicted miRNA targets from TargetScan5.1.<bold>Results: </bold>We identified 13 miRNAs for which expression levels were able to correctly predict the nature of the sample analysed (IBC vs non-IBC). For these miRNAs, we detected a total of 17,295 correlated miRNA-mRNA pairs, of which 7012 and 10 283 pairs showed negative and positive correlations, respectively. For four miRNAs (miR-29a, miR-30b, miR-342-3p and miR-520a-5p), correlated genes were concordant with predicted targets. A gene set enrichment analysis on these genes demonstrated significant enrichment in biological processes related to cell proliferation and signal transduction.<bold>Conclusions: </bold>This study represents, to the best of our knowledge, the first integrated analysis of miRNA and mRNA expression in IBC. We identified a set of 13 miRNAs of which expression differed between IBC and non-IBC, making these miRNAs candidate markers for the IBC subtype.
- Subjects
MESSENGER RNA; INFLAMMATORY breast cancer; GENE expression; PHENOTYPES; CELL proliferation; HER2 protein
- Publication
British Journal of Cancer, 2010, Vol 103, Issue 4, p532
- ISSN
0007-0920
- Publication type
journal article
- DOI
10.1038/sj.bjc.6605787