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- Title
Reducing progoitrin and enriching glucoraphanin in Braasica napus seeds through silencing of the GSL- ALK gene family.
- Authors
Liu, Zheng; Hirani, Arvind; McVetty, Peter; Daayf, Fouad; Quiros, Carlos; Li, Genyi
- Abstract
The hydrolytic products of glucosinolates in brassica crops are bioactive compounds. Some glucosinolate derivatives such as oxazolidine-2-thione from progoitrin in brassica oilseed meal are toxic and detrimental to animals, but some isothiocyanates such as sulforaphane are potent anti-carcinogens that have preventive effects on several human cancers. In most B. rapa, B. napus and B. juncea vegetables and oilseeds, there is no or only trace amount of glucoraphanin that is the precursor to sulforaphane. In this paper, RNA interference (RNAi) of the GSL- ALK gene family was used to down-regulate the expression of GSL- ALK genes in B. napus. The detrimental glucosinolate progoitrin was reduced by 65 %, and the beneficial glucosinolate glucoraphanin was increased to a relatively high concentration (42.6 μmol g seed) in seeds of B. napus transgenic plants through silencing of the GSL- ALK gene family. Therefore, there is potential application of the new germplasm with reduced detrimental glucosinolates and increased beneficial glucosinolates for producing improved brassica vegetables.
- Subjects
BRASSICA; PLANT gene silencing; GLUCOSINOLATES; ANAPLASTIC lymphoma kinase; HYDROLASES; COLE crops; OILSEEDS; ISOTHIOCYANATES; PLANT germplasm
- Publication
Plant Molecular Biology, 2012, Vol 79, Issue 1-2, p179
- ISSN
0167-4412
- Publication type
Article
- DOI
10.1007/s11103-012-9905-2