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- Title
An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation.
- Authors
Hanein, Dorit; Volkmann, Niels; Goldsmith, Sharon; Michon, Anne-Marie; Lehman, William; Craig, Roger; DeRosier, David; Almo, Steve; Matsudaira, Paul
- Abstract
Using a new procedure that combines electron-density correlation with biochemical information, we have fitted the crystal structure of the N-terminal actin-binding domain of human T-fimbrin to helical reconstructions of fimbrin-decorated actin filaments. The map locates the N-terminal calcium-binding domain and identifies actin-binding site residues on the two calponin-homology domains of fimbrin. Based on this map, we propose a model of a fimbrin crosslink in an actin bundle and its regulation by calcium.
- Subjects
ACTIN; CYTOPLASMIC filaments; ELECTRON distribution
- Publication
Nature Structural Biology, 1998, Vol 5, Issue 9, p787
- ISSN
1072-8368
- Publication type
Article
- DOI
10.1038/1828