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- Title
Chronic restraint stress promotes oral squamous cell carcinoma development by inhibiting ALDH3A1 via stress response hormone.
- Authors
Luo, Shihong; Long, Huiqing; Lou, Fangzhi; Liu, Yiyun; Wang, Haiyang; Pu, Juncai; Ji, Ping; Jin, Xin
- Abstract
Background: Chronic restraint stress (CRS) has iteratively been reported to be possibly implicated in the development of numerous cancer types. However, its role in oral squamous cell carcinoma (OSCC) has not been well elucidated. Here we intended to evaluate the role and mechanism. Methods: The effects of CRS were investigated in xenograft models of OSCC by using transcriptome sequencing, LC–MS, ELISA and RT-PCR. Moreover, the role of CRS and ALDH3A1 on OSCC cells was researched by using Trans-well, flow cytometry, western blotting, immunofluorescence, ATP activity and OCR assay. Furthermore, immunohistochemical staining was employed to observe the cell proliferation and invasion of OSCC in xenotransplantation models. Results: CRS promoted the progression of OSCC in xenograft models, stimulated the secretion of norepinephrine and the expression of ADRB2, but decreased the expression of ALDH3A1. Moreover, CRS changed energy metabolism and increased mitochondrial metabolism markers. However, ALDH3A1 overexpression suppressed proliferation, EMT and mitochondrial metabolism of OSCC cells. Conclusion: Inhibition of ALDH3A1 expression plays a pivotal role in CRS promoting tumorigenic potential of OSCC cells, and the regulatory of ALDH3A1 on mitochondrial metabolism may be involved in this process.
- Subjects
PHYSIOLOGICAL stress; BIOLOGICAL models; FLOW cytometry; REVERSE transcriptase polymerase chain reaction; ENERGY metabolism; HORMONES; XENOGRAFTS; ANIMAL experimentation; WESTERN immunoblotting; LIQUID chromatography-mass spectrometry; HEAD &; neck cancer; RISK assessment; GENE expression; RESTRAINT of patients; CELL proliferation; FLUORESCENT antibody technique; ENZYME-linked immunosorbent assay; GENE expression profiling; GENETIC markers; RESEARCH funding; SQUAMOUS cell carcinoma; PSYCHOLOGICAL stress; MICE; DISEASE risk factors
- Publication
BMC Oral Health, 2024, Vol 24, Issue 1, p1
- ISSN
1472-6831
- Publication type
Article
- DOI
10.1186/s12903-023-03787-1