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- Title
The interaction of nucleolin with G-quadruplex regions in the rotavirus genome modulates the production of viral progeny during virus replication.
- Authors
Hernández-Guzmán, Jey; López, Susana; Arias, Carlos F.; Sandoval-Jaime, Carlos
- Abstract
Cellular proteins play an important role in the replication cycle of viruses; several reports have shown a regulatory association between cellular proteins and the viral RNA. Nucleolin, a cellular protein involved in cellular RNA metabolism, has a regulatory role in the replication of hepatitis C virus, human immunodeficiency virus, and Epstein-Barr virus infection. Here, we found that the knockdown of nucleolin results in a 5-fold increased production of rotavirus infectious viral particles, and a 6-fold increased number of genome copies during virus replication. The interaction between viral RNA and nucleolin was demonstrated through co-immunoprecipitation assays, Next, to identify putative nucleolin binding sites, known as G4 sequences, in the rhesus rotavirus (RRV) genome, bioinformatic tools were used. We found three potential G4 sites on RRV gene segment 10, named G4-1 (nt 116-132), G4-2 (nt 572-607), and G4-3 (nt 717-737). Transfection experiments with these regions, showed that cells transfected with G4-1 and G4-3 regions exhibited a 64 % and 118 % increase in viral production, respectively, while G4-2 transfections had no significant effect 11.5 %. These findings suggest that nucleolin likely plays a regulatory role in controlling viral particle production through its direct interactions with G-quadruplex structures in the virus genome.
- Publication
Veterinaria México OA, 2024, Vol 11, p10
- ISSN
2448-6760
- Publication type
Article
- DOI
10.22201/fmvz.24486760e.2024.1304