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- Title
Isothermal Technologies for HPV Detection: Current Trends and Future Perspectives.
- Authors
Flores-Contreras, Elda A.; González-González, Everardo; Trujillo-Rodríguez, Gerardo de Jesús; Rodríguez-Sánchez, Iram P.; Ancer-Rodríguez, Jesús; Pérez-Maya, Antonio Alí; Alvarez-Cuevas, Salomon; Martinez-Fierro, Margarita L.; Marino-Martínez, Iván A.; Garza-Veloz, Idalia
- Abstract
The human papillomavirus (HPV) is a non-enveloped DNA virus transmitted through skin-to-skin contact that infects epithelial and mucosal tissue. It has over 200 known genotypes, classified by their pathogenicity as high-risk and low-risk categories. High-risk HPV genotypes are associated with the development of different types of cancers, including cervical cancer, which is a leading cause of mortality in women. In clinical practice and the market, the principal tests used to detect HPV are based on cytology, hybrid detection, and qPCR. However, these methodologies may not be ideal for the required timely diagnosis. Tests have been developed based on isothermal nucleic acid amplification tests (INAATs) as alternatives. These tests offer multiple advantages over the qPCR, such as not requiring specialized laboratories, highly trained personnel, or expensive equipment like thermocyclers. This review analyzes the different INAATs applied for the detection of HPV, considering the specific characteristics of each test, including the HPV genotypes, gene target, the limit of detection (LOD), detection methods, and detection time. Additionally, we discuss the tests available on the market that are approved by the Food and Drug Administration (FDA). Finally, we address the challenges and potential solutions for the large-scale implementation of INAATs, particularly in rural or underserved areas.
- Subjects
NUCLEIC acid amplification techniques; HUMAN papillomavirus; MUCOUS membranes; DNA viruses; EPITHELIUM
- Publication
Pathogens, 2024, Vol 13, Issue 8, p653
- ISSN
2076-0817
- Publication type
Article
- DOI
10.3390/pathogens13080653