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- Title
Effects and Mechanism Analysis of Vascular Endothelial Growth Factor and Salvianolic Acid B on I-Low Density Lipoprotein Permeability of the Rabbit Aortary Endothelial Cells.
- Authors
Ba, Jianming; Peng, Hu; Chen, Yanqing; Gao, Yong
- Abstract
Atherosclerosis is the common pathological basis of cardiovascular and cerebrovascular disease. This study aimed to investigate the effects of vascular endothelial growth factor (VEGF) and salvianolic acid B (SalB) on the permeability of the rabbit aortary endothelial cells (RAECs) and to figure out the possible underlying molecular mechanisms. The extravasation of I-low density lipoprotein (I-LDL) through the RAECs was significantly increased by VEGF and decreased by SalB. Meanwhile, the tight junction-associated proteins occludin and claudin-5 were found downregulated by VEGF and the caveolae structure proteins caveolin-1 and caveolin-2 upregulated, which were abolished by the infusion of SalB. In addition, a marked increase in levels of cGMP and protein kinase G-1 (PKG-1) as well as activation of nuclear factor-κB (NF-κB) p65 were found after VEGF infusion, which were attenuated by SalB. This study demonstrates that VEGF and SalB can alter the LDL permeability of the RAECs by a paracellular pathway (downregulation of occludin and claudin-5) and a transcellular pathway (upregulation of caveolin-1 and caveolin-2), in which the cGMP/PKG/NF-κB signal pathway is possibly involved. The experimental results provide a new method and basic knowledge of prevention and treatment for cardiovascular and cerebrovascular disease.
- Subjects
VASCULAR endothelial growth factors; ANTIOXIDANTS; LOW density lipoproteins; ENDOTHELIAL cells; ATHEROSCLEROSIS; LABORATORY rabbits
- Publication
Cell Biochemistry & Biophysics, 2014, Vol 70, Issue 3, p1533
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-014-0089-z