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- Title
Determination of camptothecin and 10-hydroxycamptothecin in human plasma using polymer monolithic in-tube solid phase microextraction combined with high-performance liquid chromatography.
- Authors
Yi Wen; Yi Fan; Min Zhang; Yu-Qi Feng
- Abstract
A biocompatible in-tube solid-phase microextraction (SPME) device was used for the direct and on-line extraction of camptothecin and 10-hydroxycamptothecin in human plasma. Biocompatibility was achieved through the use of a poly(methacrylic acid-ethylene glycol dimethacrylate) monolithic capillary column for extraction. Coupled to high performance liquid chromatography (HPLC) with UV detection, this on-line in-tube SPME method was successfully applied to the simultaneous determination of camptothecin and 10-hydroxycamptothecin in human plasma. The calculated detection limits for camptothecin and 10-hydroxycamptothecin were found to be 2.62 and 1.79 ng/mL, respectively. The method was linear over the range of 10-1000 ng/mL. Excellent method reproducibility was achieved, yielding RSDs of 2.49 and 1.59%, respectively. The detection limit (S/N=3) of camptothecin was found to reach 0.1 ng/mL using fluorescence detection. The proposed method was shown to cope robustly with the extraction and analysis of camptothecin and 10-hydroxycamptothecin in plasma samples.
- Subjects
CAMPTOTHECIN; PLASMA products; HIGH performance liquid chromatography; ULTRAVIOLET detectors; BIOCOMPATIBILITY
- Publication
Analytical & Bioanalytical Chemistry, 2005, Vol 382, Issue 1, p204
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-005-3194-4