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- Title
A General Approach Towards Triazole‐Linked Adenosine Diphosphate Ribosylated Peptides and Proteins.
- Authors
Liu, Qiang; Kistemaker, Hans A. V.; Bhogaraju, Sagar; Dikic, Ivan; Overkleeft, Herman S.; Van der marel, Gijsbert A.; Ovaa, Huib; Van der heden van noort, Gerbrand J.; Filippov, Dmitri V.
- Abstract
Abstract: Current methods to prepare adenosine diphosphate ribosylated (ADPr) peptides are not generally applicable due to the labile nature of this post‐translational modification and its incompatibility with strong acidic conditions used in standard solid‐phase peptide synthesis. A general strategy is presented to prepare ADPr peptide analogues based on a copper‐catalyzed click reaction between an azide‐modified peptide and an alkyne‐modified ADPr counterpart. The scope of this approach was expanded to proteins by preparing two ubiquitin ADPr analogues carrying the biological relevant α‐glycosidic linkage. Biochemical validation using Legionella effector enzyme SdeA shows that clicked ubiquitin ADPr is well‐tolerated and highlights the potential of this strategy to prepare ADPr proteins.
- Subjects
ADENOSINE diphosphate ribose; TRIAZOLES; PEPTIDES; PROTEINS; LEGIONELLA
- Publication
Angewandte Chemie, 2018, Vol 130, Issue 6, p1675
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201710527