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- Title
Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates.
- Authors
van der Valk, Anne M.; Howbrook, David N.; O'Shaughnessy, Meg C.; Sarker, Dipak K.; Baker, Simon C.; Louwrier, Ariel; Lloyd, Andrew W.
- Abstract
The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.
- Subjects
POLYMERASE chain reaction; BIOTIN; STREPTAVIDIN; POLYPROPYLENE; FLUORESCENCE; MICROBIOLOGICAL assay
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 16, p1325
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1024951322955