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- Title
Age-dependent hantavirus-specific CD8<sup>+</sup> T-cell responses in mice infected with Hantaan virus.
- Authors
Araki, K.; Yoshimatsu, K.; Lee, B.-H.; Okumura, M.; Kariwa, H.; Takashima, I.; Arikawa, J.
- Abstract
Summary. To investigate age-dependent differences in hantavirus-specific CD8+ T-cell responses, mice were inoculated with 0.1 50% newborn mouse lethal dose of Hantaan virus (HTNV) at 0, 3, 7, 14, or 35 days after birth. HTNV-specific CD8+ T cells producing gamma interferon (IFN-γ) were measured on day 30 after HTNV inoculation. Although no IFN-γ-producing HTNV-specific CD8+ T cells were detected in most of the mice inoculated with HTNV on day 0 after birth, most mice inoculated at 3, 7, 14, or 35 days had HTNV-specific CD8+ T cells. The production of tumor necrosis factor alpha (TNF-α) by IFN-γ-producing CD8+ T cells and the cytotoxic activity against HTNV-infected target cells were similar in immature and adult mice. However, the number of IFN-γ-producing HTNV-specific CD8+ T cells was significantly less in mice inoculated with HTNV at 3 days than in older mice. In addition, a strong correlation between HTNV persistence and a lack of HTNV-specific CD8+ T cells was observed. These results suggest that mice over 7 days old have the ability to induce functional HTNV-specific CD8+ T-cell responses that are indistinguishable from the responses of adult mice, and that HTNV-specific CD8+ T cells are important for clearance of HTNV.
- Subjects
HANTAVIRUSES; T cells; INTERFERONS; TUMOR necrosis factors; CELL-mediated cytotoxicity; LABORATORY mice
- Publication
Archives of Virology, 2004, Vol 149, Issue 7, p1373
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-003-0285-4