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- Title
Towards Complex Tissues Replication: Multilayer Scaffold Integrating Biomimetic Nanohydroxyapatite/Chitosan Composites.
- Authors
Palazzo, Barbara; Scialla, Stefania; Barca, Amilcare; Sercia, Laura; Izzo, Daniela; Gervaso, Francesca; Scalera, Francesca
- Abstract
This study explores an approach to design and prepare a multilayer scaffold mimicking interstratified natural tissue. This multilayer construct, composed of chitosan matrices with graded nanohydroxyapatite concentrations, was achieved through an in situ biomineralization process applied to individual layers. Three distinct precursor concentrations were considered, resulting in 10, 20, and 30 wt% nanohydroxyapatite content in each layer. The resulting chitosan/nanohydroxyapatite (Cs/n-HAp) scaffolds, created via freeze-drying, exhibited nanohydroxyapatite nucleation, homogeneous distribution, improved mechanical properties, and good cytocompatibility. The cytocompatibility analysis revealed that the Cs/n-HAp layers presented cell proliferation similar to the control in pure Cs for the samples with 10% n-HAp, indicating good cytocompatibility at this concentration, while no induction of apoptotic death pathways was demonstrated up to a 20 wt% n-Hap concentration. Successful multilayer assembly of Cs and Cs/n-HAp layers highlighted that the proposed approach represents a promising strategy for mimicking multifaceted tissues, such as osteochondral ones.
- Subjects
CHITOSAN; BIOMIMETIC materials; TISSUE scaffolds; CYTOCOMPATIBILITY; BIOACTIVE glasses; TISSUES; CELL proliferation; BIOMINERALIZATION
- Publication
Bioengineering (Basel), 2024, Vol 11, Issue 5, p471
- ISSN
2306-5354
- Publication type
Article
- DOI
10.3390/bioengineering11050471