We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Glycosaminoglycans Modulate the Angiogenic Ability of Type I Collagen-Based Scaffolds by Acting on Vascular Network Remodeling and Maturation.
- Authors
Salvante, Enrica Raffaella Grazia; Popoiu, Anca Voichita; Saxena, Amulya K.; Popoiu, Tudor Alexandru; Boia, Eugen Sorin; Cimpean, Anca Maria; Rus, Florina Stefania; Dorobantu, Florica Ramona; Chis, Monica
- Abstract
Type I collagen, prevalent in the extracellular matrix, is biocompatible and crucial for tissue engineering and wound healing, including angiogenesis and vascular maturation/stabilization as required processes of newly formed tissue constructs or regeneration. Sometimes, improper vascularization causes unexpected outcomes. Vascularization failure may be caused by extracellular matrix collagen and non-collagen components heterogeneously. This study compares the angiogenic potential of collagen type I-based scaffolds and collagen type I/glycosaminoglycans scaffolds by using the chick embryo chorioallantoic membrane (CAM) model and IKOSA digital image analysis. Two clinically used biomaterials, Xenoderm (containing type I collagen derived from decellularized porcine extracellular matrix) and a dual-layer collagen sponge (DLC, with a biphasic composition of type I collagen combined with glycosaminoglycans) were tested for their ability to induce new vascular network formation. The AI-based IKOSA app enhanced the research by calculating from stereomicroscopic images angiogenic parameters such as total vascular area, branching sites, vessel length, and vascular thickness. The study confirmed that Xenoderm caused a fast angiogenic response and substantial vascular growth, but was unable to mature the vascular network. DLC scaffold, in turn, produced a slower angiogenic response, but a more steady and organic vascular maturation and stabilization. This research can improve collagen-based knowledge by better assessing angiogenesis processes. DLC may be preferable to Xenoderm or other materials for functional neovascularization, according to the findings.
- Subjects
TISSUE scaffolds; VASCULAR remodeling; GLYCOSAMINOGLYCANS; SKIN regeneration; CHORIOALLANTOIS; CHICKEN embryos; ARTIFICIAL intelligence
- Publication
Bioengineering (Basel), 2024, Vol 11, Issue 5, p423
- ISSN
2306-5354
- Publication type
Article
- DOI
10.3390/bioengineering11050423