We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
毛木耳白色突变体的遗传规律及分子标记开发.
- Authors
黄蓉梅; 徐伟南; 丑天胜; 仝宗军; 韩星; 谢宝贵
- Abstract
Auricularia cornea is one of the most consumed edible mushrooms in China. Normally, commercial varieties of A. cornea are dark brown in colors. In 2016, combination of three kinds of fruiting bodies i.e. brown, white and white with brown patch, had been observed in A. cornea strain 43012H in a cultivating shed in Zhangzhou, China. Dikaryotic strains were isolated from these three kinds of fruiting bodies (white, brown, and variegated) and were named as AC_B (brown), AC_W (white), AC_R and AC_RW (the latter two were respectively isolated from variegated sample with brown and white patches). These isolates were inoculated separately to sawdust medium for fruiting body formation. As a result, isolate AC_B produced normal brown color fruiting bodies of A. cornea, while AC_W, AC_R and AC_RW formed white fruiting bodies. Co-culture of AC_B + AC_W, AC_B + AC_R and AC_B + AC_RW resulted in simultaneous formation of white, brown and variegated fruiting bodies in all inoculated bags. Repeated experiments obtained the same result. Genomic sequencing of AC_B and AC_W strains gained two single-nucleotide polymorphism (SNPs) sites (SNP1 and SNP2) that might be associated with the colors in fruiting bodies. Isolates with brown fruiting bodies had the genotype of heterozygous SNP1 (A/G) and homozygous SNP2 (A), while isolates with white fruiting bodies homozygous SNP1 (G) and heterozygous SNP2 (A/C).
- Publication
Mycosystema, 2018, Vol 37, Issue 12, p1643
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.180202