We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
基于16S测序分析白蜡虫二龄雌雄若虫 共生菌的多样性分析.
- Authors
吕 品; 亓 倩; 杨 璞; 陈 航; 陈晓鸣
- Abstract
[Objective] To investigate the variation and diversity of symbiotic bacteria both in the second-instar male and female of Ericerus pela. [Method] The V3-V4 region of the 16 S rRNA was performed using high-throughput sequencing technology. Uparse software was used to cluster the qualified labels. Species annotation was taken by using the Mothur and SILVA’s SSUrRNA database to analysis the differences in species abundance and diversity between male and female symbiotic bacteria. [Result] There were 1798646 qualified labels identified in this experiment. Cluster analysis was performed with 97% similarity as the standard, and 1 334 OTUs(operational taxonomic unit) were obtained. After the annotation, 14 phylums, 29 classes, 60 orders, 109 families, 165 genera, and 55 species were obtained. E. pela is significantly different from the other insects. For the second-instar nymph, Rickettsia(85.740% in female, 95.462% in male) was the dominant bacteria while Buchnera and Wolbachia were absent. A total of 386 symbiotic bacteria were identified in the second-instar nymphs. [Conclusion] The endosymbiotic bacteria of E. pela are quite different from that of other insects. Rickettsia has an absolute advantage in the second-instar nymph of E. pela. Rhizobiales and Bacillales with nitrogen fixation, Sphingomonadales which can synthesize carotenoids, are the subdominant bacteria and occupy a certain proportion in symbiotic bacteria. The unique phenomenon of the E. pela symbiotic bacteria may be related to its unique biological habits, ecological characteristics and nutrition.
- Subjects
NITROGEN fixation; SPECIES diversity; BACTERIAL diversity; RICKETTSIA; WOLBACHIA; FISH breeding
- Publication
Forest Research, 2019, Vol 32, Issue 5, p34
- ISSN
1001-1498
- Publication type
Article
- DOI
10.13275/j.cnki.lykxyj.2019.05.005