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- Title
J1-31 protein expression in astrocytes and astrocytomas.
- Authors
Shuangshoti, Shanop; Thorner, Paul S.; Ruangvejvorachai, Preecha; Saha, Baisakhi; Groshen, Susan; Taylor, Clive R.; Malhotra, Sudarshan; Imam, S. Ashraf
- Abstract
J1-31 is one of the astrocytic proteins, the expression of which has not been evaluated in astrocytomas. In the present study, we studied the expression of J1-31 protein in astrocytes and astrocytomas in comparison with GFAP, p53 and Ki-67. Materials consisted of formalin-fixed paraffin-embedded tissue specimens that included five cases of normal brain, 17 of gliosis, 15 of pilocytic astrocytoma (WHO grade I), 26 of low-grade diffuse astrocytoma (WHO grade II), four of anaplastic astrocytoma (WHO grade III), and eight of glioblastoma (WHO grade IV). GFAP was highly expressed in all specimens examined. The anti-J1-31 antibody exhibited strong cytoplasmic staining of reactive gliosis in 17/17 (100%) cases with a higher intensity of staining than that observed in the adjacent normal astrocytes. The antibody showed reactivity with tumor cells in 12/15 (80%) cases of pilocytic astrocytoma, although intensity of staining was generally weaker and more focal than observed in reactive gliosis. J1-31-positive tumor cells were detected in only 9/26 (35%) cases of the low-grade diffuse astrocytoma and none of the cases of anaplastic astrocytoma and glioblastoma. Increasing Ki-67 indices paralleled advancing tumor grades. p53 protein was expressed more commonly in infiltrating astrocytomas compared to pilocytic astrocytoma. In conclusion, down-regulation of J1-31 expression correlates with advancing grade of astrocytomas. The result suggests this protein plays some role in astrocytes that is progressively lost in malignant progression. The anti-J1-31 antibody may help further our understanding of astrocytes in disease and may be useful as an aid in the pathologic diagnosis of astrocytic lesions.
- Subjects
ASTROCYTES; ASTROCYTOMAS; GENE expression; GLIOBLASTOMA multiforme; IMMUNOGLOBULINS; WORLD Health Organization
- Publication
Neuropathology, 2009, Vol 29, Issue 5, p521
- ISSN
0919-6544
- Publication type
Article
- DOI
10.1111/j.1440-1789.2008.00979.x