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- Title
Heme Oxygenase-1 Suppresses Bovine Viral Diarrhoea Virus Replication in vitro.
- Authors
Zhang, Chong; Pu, Fengxing; Zhang, Angke; Xu, Lele; Li, Na; Yan, Yunhuan; Gao, Jiming; Liu, Hongliang; Zhang, Gaiping; Goodfellow, Ian G.; Zhou, En-Min; Xiao, Shuqi
- Abstract
Viral cycle progression depends upon host-cell processes in infected cells, and this is true for bovine viral diarrhoea virus (BVDV), the causative agent of BVD that is a worldwide threat to the bovine industry. Heme oxygenase-1 (HO-1) is a ubiquitously expressed inducible isoform of the first and rate-limiting enzyme for heme degradation. Recent studies have demonstrated that HO-1 has significant antiviral properties, inhibiting the replication of viruses such as ebola virus, human immunodeficiency virus, hepatitis C virus, and porcine reproductive and respiratory syndrome virus. However, the function of HO-1 in BVDV infection is unclear. In the present study, the relationship between HO-1 and BVDV was investigated. In vitro analysis of HO-1 expression in BVDV-infected MDBK cells demonstrated that a decrease in HO-1 as BVDV replication increased. Increasing HO-1 expression through adenoviral-mediated overexpression or induction with cobalt protoporphyrin (CoPP, a potent HO-1 inducer), pre- and postinfection, effectively inhibited BVDV replication. In contrast, HO-1 siRNA knockdown in BVDV-infected cells increased BVDV replication. Therefore, the data were consistent with HO-1 acting as an anti-viral factor and these findings suggested that induction of HO-1 may be a useful prevention and treatment strategy against BVDV infection.
- Subjects
HEME oxygenase; BOVINE viral diarrhea virus; EBOLA virus; HIV; PORCINE reproductive &; respiratory syndrome; SMALL interfering RNA
- Publication
Scientific Reports, 2015, p15575
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep15575