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- Title
Extracellular enolase of Candida albicans is involved in colonization of mammalian intestinal epithelium.
- Authors
Silva, Richard C.; Padovan, Ana Carolina B.; Pimenta, Daniel C.; Ferreira, Renata C.; da Silva, Claudio V.; Briones, Marcelo R. S.
- Abstract
Enolase is secreted by Candida albicans and is present in its biofilms although its extracellular function is unknown. Here we show that extracellular enolase mediates the colonization of small intestine mucosa by C. albicans. Assays using intestinal mucosa disks show that C. albicans adhesion is inhibited, in a dose dependent mode, either by pretreatment of intestinal epithelium mucosa disks with recombinant C. albicans enolase (70% at 0.5 mg/ml enolase) or by pretreatment of C. albicansyeasts with anti-enolase antibodies (48% with 20μg antiserum). Also using flow cytometry, immunoblots of conditioned media and confocal microscopy we demonstrate that enolase is present in biofilms and that the extracellular enolase is not an artifact due to cell lysis, but must represent functional secretion of a stable form. This is the first direct evidence that C. albicans' extracellular enolase mediates colonization on its primary translocation site. Also, because enolase is encoded by a single locus in C. albicans, its dual role peptide, as glycolytic enzyme and extracellular peptide, is a remarkable example of gene sharing in fungi.
- Subjects
CANDIDA albicans; ENOLASE; CELL adhesion -- Molecular aspects; INFECTION; INTESTINAL diseases; HYDRATASES; GENETICS; PHYSIOLOGY
- Publication
Frontiers in Cellular & Infection Microbiology, 2014, Vol 4, p1
- ISSN
2235-2988
- Publication type
Article
- DOI
10.3389/fcimb.2014.00066