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- Title
Epstein–Barr virus encoded nuclear protein EBNA-3 binds XAP-2, a protein associated with Hepatitis B virus X antigen.
- Authors
Kashuba, Elena; Kashuba, Vladimir; Pokrovskaja, Katja; Klein, George; Szekely, Laszlo
- Abstract
EBNA-3 (also called EBNA-3A) is one of the EBV encoded nuclear antigens that are necessary for B-cell transformation. EBNA-3 is known to target RBPs, nuclear proteins that also interacts with EBNA-2, EBNA-4 and EBNA-6. In order to identify additional EBNA-3 targets, an EBV-transformed human lymphocyte cDNA library was screened in the yeast two-hybrid system with N-terminus truncated EBNA-3 that cannot interact with RBP-Jκ. A clone, encoding Xap-2 protein, a cellular partner of Hepatitis B virus X-antigen was isolated. This protein is also known as the p38 subunit of the aryl hydrocarbon receptor complex (ARA9). The specific binding to EBNA-3 was confirmed by showing that the GST-Xap-2 precipitated EBNA-3 from CV1 cells that were infected with recombinant vaccinia virus expressing EBNA-3. Deletion of the C-terminus of Xap-2 eliminated the binding. Fusion with green fluorescent protein showed that Xap-2 is preferentially cytoplasmic but translocates to the nucleus upon expression of EBNA-3. Oncogene (2000) 19, 1801–1806
- Subjects
EPSTEIN-Barr virus; B cells; NUCLEAR matrix; HEPATITIS B virus
- Publication
Oncogene, 2000, Vol 19, Issue 14, p1801
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1203501