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- Title
IgG from atopic dermatitis patients induces IL‐17 and IL‐10 production in infant intrathymic TCD4 and TCD8 cells.
- Authors
Sgnotto, Fábio D. R.; de Oliveira, Marilia G.; Lira, Aline A. L.; Inoue, Amanda H. S.; Titz, Tiago O.; Orfali, Raquel L.; Bento‐de‐Souza, Luciana; Sato, Maria N.; Aoki, Valeria; Duarte, Alberto J. S.; Victor, Jefferson R.
- Abstract
Abstract: Introduction: Our group recently demonstrated that IgG modulates <italic>αβ</italic>T cell cytokine production during the maturation process in the human thymus. The effects of this modulation are IgG repertoire dependent and can exert a systemic and long‐term impact. Objective: To investigate whether IgG from atopic dermatitis (AD) patients can modulate cytokine production of infant intrathymic TCD4 and TCD8 cells <italic>in vitro</italic>. Methods: Thymic tissues were obtained from newborn children from nonatopic mothers, and thymocytes were cultured for 6 days with purified IgG from AD patients or with intravenous immunoglobulin (IVIG) or mock conditions as controls. Cells were gated as double positive T cells (TDP−CD4+CD8+), TCD4 cells (CD4+CD8−), or TCD8 cells (CD4−CD8+), and intracellular levels of IL‐17A, IFN‐<italic>γ</italic>, TNF‐<italic>α</italic>, IL‐4, IL‐10, and TGF‐<italic>β</italic> were evaluated by flow cytometry. Results: Compared to mock and IVIG culture conditions, IgG of AD individuals induced <italic>in vitro</italic> intracellular production of IL‐17 and IL‐10 by intrathymic TDP, TCD4, and TCD8 cells of infants. TGF‐<italic>β</italic> was also detected at a higher frequency in response to AD IgG in TDP and TCD8 cells compared to mock and IVIG cultured conditions. An opposite effect was detected upon IFN‐<italic>γ</italic> production in TCD4 cells, such that AD IgG reduced IFN‐<italic>γ</italic> production compared to production under mock conditions but not under IVIG conditions. Conclusion: IgG of AD patients can stimulate cytokine production in infant thymocytes and thus resembles the peripheral profile observed in adults. These findings suggest a novel mechanism that can contribute to AD pathogenesis.
- Subjects
ATOPIC dermatitis; INTRAVENOUS immunoglobulins; ATOPIC dermatitis treatment; CYTOKINE receptors; THYMOCYTES; DIAGNOSIS
- Publication
International Journal of Dermatology, 2018, Vol 57, Issue 4, p434
- ISSN
0011-9059
- Publication type
Article
- DOI
10.1111/ijd.13907