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- Title
THE MEASUREMENT OF ASYMMETRIC DIMETHYLARGININE IN HUMAN PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY.
- Authors
KURT, Yasemin Gülcan; ÖZTOSUN, Muzaffer; AYDIN, İbrahim; AKGÜL, Emin Ozgur; MACİT, Enis; AGİLLİ, Mehmet; ÇAKIR, Erdinç; ÇAYCI, Tuncer; YAMAN, Halil
- Abstract
Objective: We aimed to establish a high performance liquid chromatography (HPLC) method to measure plasma ADMA levels easily, sensitively and reliably. Methods: We used fluorescence detection, 50 mM sodium acetate buffer/ methanol/ Tetrahydrofuran as a mobile phase (A, 82:17:1; B, 22:77:1, % v, respectively), gradient method, 338 and 425 nm excitation and emission wavelengths, respectively. Samples were derivatizated with o-Phthaldialdehyde. Results: The recovery was found as 96 to 98,3 %. Intra-assay and inter-assay coefficients of variation (CV) were 2.3% and 2.7%, respectively. Conclusion: These findings suggest that our method is suitable for routine determination of ADMA more reliably.
- Subjects
ASYMMETRIC dimethylarginine; BLOOD plasma; HIGH performance liquid chromatography; HIGH pressure (Science); BLOOD
- Publication
Turkish Journal of Biochemistry / Turk Biyokimya Dergisi, 2012, Vol 37, Issue S1, p384
- ISSN
0250-4685
- Publication type
Article