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- Title
Persister-promoting bacterial toxin TisB produces anion-selective pores in planar lipid bilayers
- Authors
Gurnev, Philip A.; Ortenberg, Ron; Dörr, Tobias; Lewis, Kim; Bezrukov, Sergey M.
- Abstract
Abstract: We studied membrane activity of the bacterial peptide TisB involved in persister cell formation. TisB and its analogs form multi-state ion-conductive pores in planar lipid bilayers with all states displaying similar anionic selectivity. TisB analogs differing by ±1 elementary charges show corresponding changes in selectivity. Probing TisB pores with poly-(ethylene glycol)s reveals only restricted partitioning even for the smallest polymers, suggesting that the pores are characterized by a relatively small diameter. These findings allow us to suggest that TisB forms clusters of narrow pores that are essential for its mechanism of action.
- Subjects
BACTERIAL toxins; ANIONS; BACTERIAL cell membranes; BACTERIAL lipids; BIOCHEMICAL mechanism of action; IONS
- Publication
FEBS Letters, 2012, Vol 586, Issue 16, p2529
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.06.021