We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Dietary Polyunsaturated Fatty Acids Alter Myocardial Protein Kinase C Expression and Affect Cardioprotection Induced by Chronic Hypoxia.
- Authors
Hlaváčková, Markéta; Neckář, Jan; Ježková, Jana; Balková, Patricie; Staňková, Barbora; Nováková, Olga; Kolář, František; Novák, František
- Abstract
We examined the influence of dietary fatty acid (FA) classes on the expression of protein kinase C (PKC) δ and ε in relation to the cardioprotective effects of chronic intermittent hypoxia (CIH). Adult male Wistar rats were fed a nonfat diet enriched with 10% lard (saturated FA [SFA]), fish oil (n-3 polyunsaturated FA [n-3 PUFA]), or corn oil (n-6 PUFA) for 10 weeks. After 4 weeks on the diet, each group was divided into two subgroups that were either exposed to CIH in a barochamber (7000 m, 8 hrs/ day) or kept at normoxia for an additional 5–6 weeks. A FA phospholipid profile and Western blot analysis of PKC were performed in left ventricles. Infarct size was assessed in anesthetized animals subjected to 20-min coronary artery occlusion and 3-hr reperfusion. CIH decreased the n-6/n-3 PUFA ratio in all groups by 23% independently of the initial value set by various diets. The combination of n-3 diet and CIH had a stronger antiarrhythmic effect during reperfusion than the n-3 diet alone; this effect was less pronounced in rats fed the n-6 diet. The normoxic n-6 group exhibited smaller infarctions (by 22%) than the n-3 group. CIH decreased the infarct size in n-3 and SFA groups (by 20% and 23%, respectively) but not in the n-6 group. Unlike PKCε, the abundance of PKCδ in the myocardial particulate fraction was increased by CIH except for the n-6 group. Myocardial infarct size was negatively correlated (r = − 0.79) with the abundance of PKCδ in the particulate fraction. We conclude that lipid diets modify the infarct size–limiting effect of CIH by a mechanism that involves the PKCδ-dependent pathway.
- Publication
Experimental Biology & Medicine, 2007, Vol 232, Issue 6, p823
- ISSN
1535-3702
- Publication type
Article
- DOI
10.3181/00379727-232-2320823