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- Title
Genetic diversity and population structure of Melia azedarach in North-Western Plains of India.
- Authors
Thakur, Sapna; Choudhary, Shruti; Singh, Amandeep; Ahmad, Kamal; Sharma, Gagan; Majeed, Aasim; Bhardwaj, Pankaj
- Abstract
Key message : Genetic structure among M. azedarach populations was detected and two subpopulations were present among them. A significant 'isolation by distance' was found in M. azedarach population in North-Western Plains of India. Abstract: Melia azedarach is an important forest tree with pharmaceutical, insecticidal, pesticidal, and commercial significance. It is a good reforestation tree because of its fast growth and drought hardy nature. Genetic variation in a species allows itself to adapt, evolve and respond to environmental stress. It provides the basis for survival of a species and critically influences its evolutionary potential. Assessment of genetic diversity is necessary for improvement and conservation of a species. For this, microsatellite markers are of particular interest given the attributes like co-dominance, reproducibility, hyper variability and abundance throughout the genome. In the present study, we analyzed the genetic diversity and population structure of M. azedarach, an ecologically imperative species growing in the North-Western Plains of India. We developed 43 microsatellite markers, of which 20 were subsequently employed for analysis of diversity and population structure among 33 populations encompassing 318 genotypes representing North-Western Plains of India. A moderate level of diversity (Na = 5.1, Ho = 0.506, He = 0.712, I = 1.386) was assessed. The highest value of Δ K estimated using STRUCTURE indicated 2 subpopulations ( K = 2). AMOVA exhibited 73 % variation within populations and 12 % variation was found among regions. Significant positive correlation between geographical and genetic distance was found ( Rxy = 0.365, P = 0.010). The present study lays a foundation on a better understanding of genetic dynamics of the species and reveals its diversity and population structure in North-Western Plains of India.
- Subjects
MELIA; MEDICINAL plants; PLANT genetics; PLANT populations; MICROSATELLITE repeats
- Publication
Trees: Structure & Function, 2016, Vol 30, Issue 5, p1483
- ISSN
0931-1890
- Publication type
Article
- DOI
10.1007/s00468-016-1381-x