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- Title
Distinct Subsets of Syt-IV/BDNF Vesicles Are Sorted to Axons versus Dendrites and Recruited to Synapses by Activity.
- Authors
Dean, Camin; Liu, Huisheng; Staudt, Thorsten; Stahlberg, Markus A.; Vingill, Siv; Bückers, Johanna; Kamin, Dirk; Engelhardt, Johann; Jackson, Meyer B.; Hell, Stefan W.; Chapman, Edwin R.
- Abstract
BDNF plays a critical role in the regulation of synaptic strength and is essential for long-term potentiation, a phenomenon that underlies learning and memory. However, whether BDNF acts in a diffuse manner or is targeted to specific neuronal subcompartments or synaptic sites to affect circuit function remains unknown. Here, using photoactivation of BDNF or syt-IV (a regulator of exocytosis present on BDNF-containing vesicles) in transfected rat hippocampal neurons, we discovered that distinct subsets of BDNF vesicles are targeted to axons versus dendrites and are not shared between these compartments. Moreover, syt-IV- and BDNF-harboring vesicles are recruited to both presynaptic and postsynaptic sites in response to increased neuronal activity. Finally, using syt-IV knockout mouse neurons, we found that syt-IV is necessary for both presynaptic and postsynaptic scaling of synaptic strength in response to changes in network activity. These findings demonstrate that BDNF-containing vesicles can be targeted to specific sites in neurons and suggest that syt-IVregulated BDNF secretion is subject to spatial control to regulate synaptic function in a site-specific manner.
- Subjects
SYNAPTOTAGMINS; BRAIN-derived neurotrophic factor; AXONS; DENDRITES; SYNAPSES; EXOCYTOSIS; PRESYNAPTIC receptors
- Publication
Journal of Neuroscience, 2012, Vol 32, Issue 16, p5398
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.4515-11.2012