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- Title
Human Respiratory Syncytial Virus-Induced Immune Signature of Infection Revealed by Transcriptome Analysis of Clinical Pediatric Nasopharyngeal Swab Samples.
- Authors
Lamballerie, Claire Nicolas De; Pizzorno, Andrés; Dubois, Julia; Padey, Blandine; Julien, Thomas; Traversier, Aurélien; Carbonneau, Julie; Orcel, Elody; Lina, Bruno; Hamelin, Marie-Eve; Roche, Magali; Textoris, Julien; Boivin, Guy; Legras-Lachuer, Catherine; Terrier, Olivier; Rosa-Calatrava, Manuel; Nicolas De Lamballerie, Claire
- Abstract
Human respiratory syncytial virus (HRSV) constitutes one the main causes of respiratory infection in neonates and infants worldwide. Transcriptome analysis of clinical samples using high-throughput technologies remains an important tool to better understand virus-host complex interactions in the real-life setting but also to identify new diagnosis/prognosis markers or therapeutics targets. A major challenge when exploiting clinical samples such as nasal swabs, washes, or bronchoalveolar lavages is the poor quantity and integrity of nucleic acids. In this study, we applied a tailored transcriptomics workflow to exploit nasal wash samples from children who tested positive for HRSV. Our analysis revealed a characteristic immune signature as a direct reflection of HRSV pathogenesis and highlighted putative biomarkers of interest such as IP-10, TMEM190, MCEMP1, and TIMM23.
- Subjects
RESPIRATORY syncytial virus; NUCLEIC acids; RESPIRATORY infections; NASAL mucosa
- Publication
Journal of Infectious Diseases, 2021, Vol 223, Issue 6, p1052
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiaa468