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- Title
Biocompatibility and surface structure of chemically modified immunoisolating alginate-PLL capsules.
- Authors
C. M. Bünger; C. Gerlach; T. Freier; K. P. Schmitz; M. Pilz; C. Werner; L. Jonas; W. Schareck; U. T. Hopt; P. de Vos
- Abstract
Grafting of encapsulated living cells has the potential to cure a wide variety of diseases. Large-scale application of the technique, however, is hampered by insufficient biocompatibility of the capsules. A major factor in the biocompatibility of capsules is inadequate covering of the inflammatory poly-L-lysine (PLL) on the capsules' surface. In the present study, we investigate whether tissue responses against alginate-PLL capsules can be reduced by crosslinking the surface of the capsules with heparin or polyacrylic acid. Our transplant study in rats shows a tissue response composed of fibroblasts and macrophages on alginate-PLL-alginate and alginate-PLL-heparin capsules that was completely absent on alginate-PLL-polyacrylic acid capsules. Atomic force microscopy analyses of the capsules demonstrates that the improved biocompatibility of alginate-PLL-capsules by polyacrylic acid coating should not only be explained by a more adequate binding of PLL but also by the induction of a smoother surface. This study shows for the first time that biologic responses against capsules can be successfully deleted by chemically crosslinking biocompatible molecules on the surface of alginate-PLL capsules. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 12191227, 2003
- Subjects
TRANSPLANTATION of organs, tissues, etc.; CELLS; BIOCOMPATIBILITY; HEPARIN
- Publication
Journal of Biomedical Materials Research, Part A, 2003, Vol 67, Issue 4, p1219
- ISSN
1549-3296
- Publication type
Article