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- Title
Detecting immunoglobulins in processed sputa.
- Authors
Cass, Steven P; McGrath, Joshua J.C.; Son, Kiho; Radford, Katherine; LaVigne, Nicola; Klein, Ditte K; Ditlev, Sisse B; Porsbjerg, Celeste; Nair, Parameswaran; Stampfli, Martin R.; Mukherjee, Manali
- Abstract
However, DTT increased the recovery of total IgA, and a disease-relevant IgG auto-antibody,3 while reducing IgE yield. In summary, we report that PBS/DTT sputum processing buffers are comparable in terms of IgG and IgM recovery. GLO:1X5/01dec21:all15049-fig-0002.jpg PHOTO (COLOR): 2 Dithiothreitol processing moderately improves sputum IgG and IgA recovery but reduces IgE.
- Subjects
IMMUNOGLOBULINS; SPUTUM; CHRONIC obstructive pulmonary disease
- Publication
Allergy, 2021, Vol 76, Issue 12, p3798
- ISSN
0105-4538
- Publication type
Article
- DOI
10.1111/all.15049