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- Title
Activation by vanadate of glycolysis in hepatocytes from diabetic rats.
- Authors
Rodríguez-Gil, Joan E.; Gómez-Foix, Anna M.; Fillat, Cristina; Bosch, Fàtima; Guinovart, Joan J.; Rodríguez-Gil, J E; Gómez-Foix, A M; Fillat, C; Bosch, F; Guinovart, J J
- Abstract
In hepatocytes from starved streptozocin-induced diabetic rats, vanadate increases the glycolytic flux because it raises the levels of fructose-2,6-bisphosphate (Fru-2,6-P2), the main regulatory metabolite of this pathway. This effect of vanadate on Fru-2,6-P2 levels is time and dose dependent, and it remains in cells incubated in a calcium-depleted medium. Vanadate is also able to counteract the decrease on Fru-2,6-P2 levels produced by glucagon, colforsin, or exogenous cAMP. However, vanadate does not modify 6-phosphofructo-2-kinase and pyruvate kinase activities, but it does counteract the inactivation of these enzymes induced by glucagon. Likewise, Fru-2,6-P2ase activity is also not affected by vanadate. In addition, vanadate is able to increase the production of both lactate and CO2 in hepatocytes from streptozocin-induced diabetic rats incubated in the presence of glucose in the medium. Vanadate behaves as a glycolytic effector in these cells, and this effect may be related to its ability to normalize blood glucose levels in diabetic animals.
- Publication
Diabetes, 1991, Vol 40, Issue 10, p1355
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diab.40.10.1355