We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
LNK promotes the growth and metastasis of triple negative breast cancer via activating JAK/STAT3 and ERK1/2 pathway.
- Authors
Lv, Jianxin; Yu, Wei; Zhang, Yanan; Cao, Xinhua; Han, Lifei; Hu, Haolin; Wang, Chenfei
- Abstract
Background: LNK adaptor protein is a crucial regulator of normal hematopoiesis, which down-regulates activated tyrosine kinases at the cell surface resulting in an antitumor effect. To date, little studies have examined activities of LNK in solid tumors except ovarian cancer. Methods: Clinical tissue chips were obtained from 16 clinical patients after surgery. Western blotting assay and quantitative real time PCR was performed to measure the expression of LNK. We investigate the in vivo and vitro effect of LNK in Triple Negative Breast Cancer by using cell proliferation、migration assays and an in vivo murine xenograft model. Western blotting assay was performed to investigate the mechanism of LNK in triple negative breast cancer. Results: We found that the levels of LNK expression were elevated in high grade triple-negative breast cancer through Clinical tissue chips. Remarkably, overexpression of LNK can promote breast cancer cell proliferation and migration in vivo and vitro, while silencing of LNK show the opposite phenomenon. We also found that LNK can promote breast cancer cell to proliferate and migrate via activating JAK/STAT3 and ERK1/2 pathway. Conclusions: Our results suggest that the adaptor protein LNK acts as a positive signal transduction modulator in TNBC.
- Subjects
TRIPLE-negative breast cancer; ADAPTOR proteins; CANCER cell proliferation; CANCER cell migration; OVARIAN cancer; XENOGRAFTS; OVARIAN function tests
- Publication
Cancer Cell International, 2020, Vol 20, Issue 1, p1
- ISSN
1475-2867
- Publication type
Article
- DOI
10.1186/s12935-020-01197-9