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- Title
Porcine placenta hydrolysates regulate calcium disturbance in MC3T3-E1 osteoblastic cells.
- Authors
Hwa-Young Lee; Hyung-Ryong Kim; Sun-Young Park; Han-Jung Chae; Jong-Hyun Kim
- Abstract
Background: In bone metabolism, Ca2+ disturbance and oxidative damage are the main biochemical factors related to pathology. Osteoblasts are bone-forming cells that also control bone endocrinology. Endocrine hormones and proteins are matured, folded, and secreted in the endoplasmic reticulum (ER). ER stress has emerged as a new pathological mechanism to explain bone disturbance. Here we studied the role of porcine placenta hydrolysates (PPHs) in the regulation of ER stress. Methods: Cell viability was determined in vitro using trypan blue dye exclusion. ER stress and apoptosis were evaluated using immunoblotting and a caspase kit. The fluorescent Ca2+-binding dye Fura-2/AM was used to measure changes in intracellular Ca2+ ([Ca2+]i). ROS levels, NADPH oxidase activity, and superoxide dismutase (SOD) activity were also measured. Results: PPHs protected MC3T3-E1 osteoblastic cells against thapsigargin (Tg)-induced ER stress. Moreover, PPHs regulated caspase-12 and -3 activities, thereby protecting against cell death, and also regulated Tg-induced Ca2+ release. The Ca chelator BAPT/AM also regulated caspase-12 and -3 activities and prevented Ca² stress-induced cell death. In the presence of PPHs or BAPTA/AM, Ca2+-related ROS were also regulated, as demonstrated by alterations in NADPH oxidase and SOD activity. Conclusions: PPHs appear to regulate bone metabolism disturbance by controlling Ca2+ concentrations, and thus ER stress and ROS, in osteoblasts cultured in vitro.
- Subjects
BONE metabolism; CALCIUM metabolism; APOPTOSIS; IMMUNOBLOTTING; OSTEOPOROSIS; RESEARCH funding; PSYCHOLOGICAL stress; T-test (Statistics); OSTEOBLASTS; CLASSIFICATION
- Publication
BMC Complementary & Alternative Medicine, 2016, Vol 16, p1
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/s12906-016-1202-1