We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Increased expression of microRNA-301a in nonsmall-cell lung cancer and its clinical significance.
- Authors
You-kui Shi; Quan-ling Zang; Gui-xin Li; Yan Huang; Shi-zhong Wang; Shi, You-Kui; Zang, Quan-Ling; Li, Gui-Xin; Huang, Yan; Wang, Shi-Zhong
- Abstract
<bold>Aims: </bold>Recently, accumulating evidence indicates that dysregulation of microRNAs is associated with the initiation and progression of cancer. Oncogenic miR-301a has been reported upregulation and associated with tumorigenesis and progression in various types of cancer. The aim of this study was to investigate the expression of miR-301a in nonsmall-cell lung cancer. (NSCLC), and to assess its association with malignancy, metastasis and prognosis.<bold>Subjects and Methods: </bold>total of 88 NSCLC patients (females = 21 and males = 67), aged 15-81 years were included in the study. miR-301a expression in tumor tissue was estimated by real-time quantitative reverse transcription polymerase chain reaction.<bold>Results: </bold>miR-301a was significantly upregulated in NSCLC tissues compared with their paired adjacent nontumor tissues. (P < 0.001). Increased expression of miR-301a was detected in tumors with lymph node metastases. (P =0.003). In addition, high miR-301a expression was significantly associated with poorly differentiation. (P =0.015), lymph node metastasis. (P =0.013) and advanced tumor-node-metastasis. (TNM) stage. (P =0.018). A. comparison of survival curves of low versus high expressers of miR-301a revealed a highly significant difference in NSCLC, which suggests that overexpression of miR-301a is associated with a poorer disease-free survival (DFS) (P =0.002). Moreover, multivariate Cox proportional hazard regression analyses revealed that the miR-301a overexpression was an unfavorable prognostic factor for disease-free survival in addition to TNM stage.<bold>Conclusions: </bold>miR-301a may represent a novel prognostic indicator, a biomarker for the early detection of lymph node metastasis and a therapeutic target in NSCLC.
- Subjects
NON-small-cell lung carcinoma; MICRORNA; CANCER invasiveness; REVERSE transcriptase polymerase chain reaction; LYMPH nodes; DIAGNOSIS; LUNG cancer diagnosis; GENES; LUNG cancer; LUNG tumors; METASTASIS; PROGNOSIS; RNA; TUMOR classification; DISEASE progression; KAPLAN-Meier estimator; TUMOR grading
- Publication
Journal of Cancer Research & Therapeutics, 2016, Vol 12, Issue 2, p693
- ISSN
0973-1482
- Publication type
journal article
- DOI
10.4103/0973-1482.146130