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- Title
Effect of Enhanced Prostacyclin Synthesis by Adenovirus-Mediated Transfer on Lipopolysaccharide Stimulation in Neuron-Glia Cultures.
- Authors
TSAI, MAY‐JYWAN; SHYUE, SONG‐KUN; WENG, CHING‐FENG; CHUNG, YING; LIOU, DANN‐YING; HUANG, CHI‐TING; KUO, HUAI‐SHENG; LEE, MENG‐JEN; CHANG, PEI‐TEH; HUANG, MING‐CHAO; HUANG, WEN‐CHENG; LIOU, K D; CHENG, HENRICH
- Abstract
Prostacyclin (PGI2) is known as a short-lived, potent vasodilator and platelet anti-aggregatory eicosanoid. This work attempts to selectively augment PGI2 synthesis in neuron-glia cultures by adenoviral (Ad) gene transfer of PGI synthase (PGIS) or bicistronic cyclooxygenase 1 (COX-1)/PGIS and examines whether PGI2 confers protection against lipopolysaccharide (LPS) stimulation. Cultures released low levels of eicosanoids. Upon Ad-PGIS or Ad- COX-1/PGIS infection, cultures selectively increased prostacyclin release. Both PGIS- and COX-1/PGIS-overexpressed cultures contained fewer microglial numbers. Further, they significantly attenuated LPS-induced iNOS expression and lactate, nitric oxide, and TNF- production. Taken together, enhanced prostacyclin synthesis in neuron-glial cultures reduced microglia number and suppressed LPS stimulation.
- Subjects
PROSTACYCLIN; BIOSYNTHESIS; GENETIC transformation; EICOSANOIDS; NITRIC oxide
- Publication
Annals of the New York Academy of Sciences, 2005, Vol 1042, Issue 1, p338
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1196/annals.1338.031