We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Monoterpene Indole Alkaloids from the Aerial Parts of Rhazya stricta Induce Cytotoxicity and Apoptosis in Human Adenocarcinoma Cells.
- Authors
Abdul-Hameed, Zainab H.; Bawakid, Nahed O.; Alorfi, Hajer S.; Sobahi, Tariq R.; Alburae, Najla Ali; Abdel-Lateff, Ahmed; Elbehairi, Serag Eldin I.; Alfaifi, Mohammad Y.; Alhakamy, Nabil A.; Alarif, Walied M.
- Abstract
Chromatographic investigation of the aerial parts of the Rhazya stricta (Apocynaceae) resulted in the isolation of two new monoterpene indole alkaloids, 6-nor-antirhine-N1-methyl (1) and razyamide (2), along with six known compounds, eburenine (3), epi-rhazyaminine (4), rhazizine (5), 20-epi-sitsirikine (6), antirhine (7), and 16-epi-stemmadenine-N-oxide (8). The chemical structures were established by various spectroscopic experiments. Compounds 1–8 exhibited cytotoxic effects against three cancer cells with IC50 values ranging between 5.1 ± 0.10 and 93.2 ± 9.73 µM against MCF-7; 5.1 ± 0.28 and 290.2 ± 7.50 µM against HepG2, and 3.1 ± 0.17 and 55.7 ± 4.29 µM against HeLa cells. Compound 2 showed the most potent cytotoxic effect against all cancer cell lines (MCF-7, HepG2 and HeLa with IC50 values = 5.1 ± 0.10, 5.1 ± 0.28, and 3.1 ± 0.17 µM, respectively). Furthermore, compound 2 revealed a significant increase in the apoptotic cell population of MCF-7, HepG2, and HeLa cells, with 31.4 ± 0.2%, 29.2 ± 0.5%, and 34.9 ± 0.6%, respectively. Compound 2 decreased the percentage of the phagocytic pathway on HepG2 cells by 15.0 ± 0.1%. These findings can explain the antiproliferative effect of compound 2.
- Subjects
INDOLE alkaloids; CELL populations; CANCER cells; APOPTOSIS; HELA cells; ADENOCARCINOMA; INDOLE; PYRROLIZIDINES
- Publication
Molecules, 2022, Vol 27, Issue 4, p1422
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules27041422