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- Title
Genotoxic and Cytotoxic Properties of Zinc Oxide Nanoparticles Phyto-Fabricated from the Obscure Morning Glory Plant Ipomoea obscura (L.) Ker Gawl.
- Authors
Murali, Mahadevamurthy; Anandan, Satish; Ansari, Mohammad Azam; Alzohairy, Mohammad A.; Alomary, Mohammad N.; Asiri, Sarah Mousa Maadi; Almatroudi, Ahmad; Thriveni, M. C.; Singh, Sudarshana Brijesh; Gowtham, Hittanahallikoppal Gajendramurthy; Aiyaz, Mohammed; Srinivasa, Chandrashekar; Urooj, Asna; Amruthesh, Kestur Nagaraj; Micale, Nicola
- Abstract
The study was undertaken to investigate the antioxidant, genotoxic, and cytotoxic potentialities of phyto-fabricated zinc oxide nanoparticles (ZnO-NPs) from Ipomoea obscura (L.) Ker Gawl. aqueous leaf extract. The UV-visible spectral analysis of the ZnO-NPs showed an absorption peak at 304 nm with a bandgap energy of 3.54 eV, which are characteristics of zinc nanoparticles. Moreover, the particles were of nano-size (~24.26 nm) with 88.11% purity and were agglomerated as observed through Scanning Electron Microscopy (SEM). The phyto-fabricated ZnO-NPs offered radical scavenging activity (RSA) in a dose-dependent manner with an IC50 of 0.45 mg mL−1. In addition, the genotoxicity studies of ZnO-NPs carried out on onion root tips revealed that the particles were able to significantly inhibit the cell division at the mitotic stage with a mitotic index of 39.49%. Further, the cytotoxic studies on HT-29 cells showed that the phyto-fabricated ZnO-NPs could arrest the cell division as early as in the G0/G1 phase (with 92.14%) with 73.14% cells showing early apoptotic symptoms after 24 h of incubation. The results of the study affirm the ability of phyto-fabricated ZnO-NPs from aqueous leaf extract of I. obscura is beneficial in the cytotoxic application.
- Subjects
IPOMOEA; ZINC oxide; CELL division; SCANNING electron microscopy; NANOPARTICLES; ONIONS; MORNING
- Publication
Molecules, 2021, Vol 26, Issue 4, p891
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules26040891