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- Title
Identification of Potential Auxin-Responsive Small Signaling Peptides through a Peptidomics Approach in Arabidopsis thaliana.
- Authors
Luo, Weigui; Xiao, Yuan; Liang, Qiwen; Su, Yi; Xiao, Langtao
- Abstract
Small signaling peptides (SSPs) are a class of short peptides playing critical roles in plant growth and development. SSPs are also involved in the phytohormone signaling pathway. However, identification of mature SSPs is still a technical challenge because of their extremely low concentrations in plant tissue and complicated interference by many other metabolites. Here, we report an optimized protocol to extract SSPs based on protoplast extraction and to analyze SSPs based on tandem mass spectrometry peptidomics. Using plant protoplasts as the material, soluble peptides were directly extracted into phosphate buffer. The interference of non-signaling peptides was significantly decreased. Moreover, we applied the protocol to identify potential SSPs in auxin treated wild type and auxin biosynthesis defective mutant yuc2yuc6. Over 100 potential SSPs showed a response to auxin in Arabidopsis thaliana.
- Subjects
SIGNAL peptides; ARABIDOPSIS thaliana; TANDEM mass spectrometry; PLANT protoplasts; DRUG residues; PLANT cells &; tissues; PLANT growth
- Publication
Molecules, 2019, Vol 24, Issue 17, p3146
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules24173146