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- Title
Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites.
- Authors
Shu-Huei Yu; Shao-Jung Wu; Jui-Yu Wu; Chih-Kang Peng; Fwu-Long Mi
- Abstract
Bioactive composites that enable the formation of calcium phosphates have received increased attention over the last decade, in the development of osteoconductive biomaterials for orthopaedic applications. In this work, tripolyphosphate (TPP)-cross-linked chitosan/gelatin composites (TPP-CG) were prepared for the growth of shape- and size-controlled calcium phosphates on/in the composites. The mineralization pattern of the composites, after soaking in the Ca(OH)2 aqueous solution, clearly demonstrated oriented, needle-like nanocrystallites of calcium phosphates in the matrix with especially high Ca/P molar ratio (3.98) as detected by energy dispersive X-ray spectroscopy (EDX) analysis. Subsequent to mineralization in a simulated body fluid (SBF), the mineralized composites showed micro-scaled spherical aggregates deposited on the surface and granule-like nanocrystallites grew in the matrix. The Ca/P molar ratio (1.72) and X-ray diffraction pattern of the nanocrystallites grown in the composites were similar to those of hydroxyapatite (HAp). Osteoblastic differentiation of ROS cells cultured on the mineralized composites allowed an enhanced expression of the chosen osteogenic marker (alkaline phosphatase, ALPase). These results indicated that the composites mineralized with micro- and nano-scaled calcium phosphates with various structural features make them attractive for bone tissue engineering applications.
- Subjects
CHITOSAN; GELATIN; APATITE in the body; CALCIUM phosphate; BIOMINERALIZATION; ALKALINE phosphatase; BONE substitutes; TISSUE engineering
- Publication
Molecules, 2013, Vol 18, Issue 1, p27
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules18010027