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- Title
Molecular mechanisms of ovarian hyperstimulation syndrome: paracrine reduction of endothelial claudin 5 by hCG in vitro is associated with increased endothelial permeability.
- Authors
M. Rodewald; D. Herr; W.C. Duncan; H.M. Fraser; G. Hack; R. Konrad; F. Gagsteiger; R. Kreienberg; C. Wulff
- Abstract
BACKGROUND Ovarian hyperstimulation syndrome (OHSS) is a potentially life-threatening complication of ovarian stimulation associated with severe vascular hyperpermeability. Primary co-cultures of human luteinized granulosa cells (LGCs) and human umbilical vein endothelial cells (HUVECs) were used as a model of steroidgenic/endothelial cell interaction in OHSS. METHODS hCG and the vascular endothelial growth factor (VEGF) inhibitor, Flt-1Fc, were added to co-cultures of LGCs and HUVECs separated by a micropore membrane. Endothelial permeability to labeled bovine serum albumin was measured and the expression of the endothelial cell-specific adhesion protein claudin 5 was investigated using immunocytochemistry and western blotting. RESULTS The addition of hCG increased HUVEC permeability in the presence of LGCs (P P P P P CONCLUSIONS For OHSS, this novel paradigm suggests that hCG can increase endothelial permeability by up-regulating VEGF in LGCs which causes reduction in endothelial claudin 5 expression.
- Subjects
OVARIAN diseases; PARACRINE mechanisms; CELL adhesion molecules; ENDOTHELIUM; CHORIONIC gonadotropins; CELL permeability; VASCULAR endothelial growth factor antagonists; GENE expression
- Publication
Human Reproduction, 2009, Vol 24, Issue 5, p1191
- ISSN
0268-1161
- Publication type
Article
- DOI
10.1093/humrep/den479