We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The clock in growing hyphae and their synchronization in Neurospora crassa.
- Authors
Cheong, Jia Hwei; Qiu, Xiao; Liu, Yang; Krach, Emily; Guo, Yinping; Bhusal, Shishir; Schüttler, Heinz-Bernd; Arnold, Jonathan; Mao, Leidong
- Abstract
Utilizing a microfluidic chip with serpentine channels, we inoculated the chip with an agar plug with Neurospora crassa mycelium and successfully captured individual hyphae in channels. For the first time, we report the presence of an autonomous clock in hyphae. Fluorescence of a mCherry reporter gene driven by a clock-controlled gene-2 promoter (ccg-2p) was measured simultaneously along hyphae every half an hour for at least 6 days. We entrained single hyphae to light over a wide range of day lengths, including 6,12, 24, and 36 h days. Hyphae tracked in individual serpentine channels were highly synchronized (K = 0.60-0.78). Furthermore, hyphae also displayed temperature compensation properties, where the oscillation period was stable over a physiological range of temperatures from 24 °C to 30 °C (Q10 = 1.00-1.10). A Clock Tube Model developed could mimic hyphal growth observed in the serpentine chip and provides a mechanism for the stable banding patterns seen in race tubes at the macroscopic scale and synchronization through molecules riding the growth wave in the device. This study utilizes a serpentine microfluidic chip to report the presence of an autonomous clock in Neurospora crassa hyphae.
- Subjects
NEUROSPORA crassa; SYNCHRONIZATION; REPORTER genes; SERPENTINE
- Publication
Communications Biology, 2024, Vol 7, Issue 1, p1
- ISSN
2399-3642
- Publication type
Article
- DOI
10.1038/s42003-024-06429-6