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- Title
Phospholipase Cδ4 is required for Ca[sup 2+] mobilization essential for acrosome reaction in sperm.
- Authors
Fukami, Kiyoko; Yoshida, Manaba; Inoue, Taakfumi; Kurokawa, Manabu; Fissore, Rafael A.; Yoshida, Nobuaki; Mikoshiba, Katsuhiko; Takenawa, Tadaomi
- Abstract
Zona pellucida (ZP)-induced acrosome reaction in sperm is a required step for mammalian fertilization. However, the precise mechanism of the acrosome reaction remains unclear. We previously reported that PLCδ4 is involved in the ZP-induced acrosome reaction in mouse sperm. Here we have monitored Ca[sup 2+] responses in single sperm, and we report that the [Ca[sup 2+]][sub i] increase in response to ZP, which is essential for driving the acrosome reaction in vivo, is absent in PLCδ4[sup -/-] sperm. Progesterone, another physiological inducer of the acrosome reaction, failed to induce sustained [Ca[sup 2+]][sub i] increases in PLCδ4[sup -/-] sperm, and consequently the acrosome reaction was par- tially inhibited. In addition, we observed oscillatory [Ca[sup 2+]][sub i] increases in wild-type sperm in response to these acrosome inducers. Calcium imaging studies revealed that the [Ca[sup 2+]][sub i] increases induced by exposure to ZP and progesterone started at different sites within the sperm head, indicating that these agonists induce the acrosome reaction via different Ca[sup 2+] mechanisms. Furthermore, store-operated channel (SOC) activity was severely impaired in PLCδ4[sup -/-] sperm. These results indicate that PLCδ4 is an important enzyme for intracellular [Ca[sup 2+]][sub i] mobilization in the ZP-induced acrosome reaction and for sustained [Ca[sup 2+]][sub i] increases through SOC induced by ZP and progesterone in sperm.
- Subjects
PHOSPHOLIPASE C; CALCIUM ions; SPERMATOZOA
- Publication
Journal of Cell Biology, 2003, Vol 161, Issue 1, p79
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200210057