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- Title
A Yellow Fever Virus 17D Infection and Disease Mouse Model Used to Evaluate a Chimeric Binjari-Yellow Fever Virus Vaccine.
- Authors
Yan, Kexin; Vet, Laura J.; Tang, Bing; Hobson-Peters, Jody; Rawle, Daniel J.; Le, Thuy T.; Larcher, Thibaut; Hall, Roy A.; Suhrbier, Andreas
- Abstract
Despite the availability of an effective, live attenuated yellow fever virus (YFV) vaccine (YFV 17D), this flavivirus still causes up to ≈60,000 deaths annually. A number of new approaches are seeking to address vaccine supply issues and improve safety for the immunocompromised vaccine recipients. Herein we describe an adult female IFNAR-/- mouse model of YFV 17D infection and disease that recapitulates many features of infection and disease in humans. We used this model to evaluate a new YFV vaccine that is based on a recently described chimeric Binjari virus (BinJV) vaccine technology. BinJV is an insect-specific flavivirus and the chimeric YFV vaccine (BinJ/YFV-prME) was generated by replacing the prME genes of BinJV with the prME genes of YFV 17D. Such BinJV chimeras retain their ability to replicate to high titers in C6/36 mosquito cells (allowing vaccine production), but are unable to replicate in vertebrate cells. Vaccination with adjuvanted BinJ/YFV-prME induced neutralizing antibodies and protected mice against infection, weight loss and liver pathology after YFV 17D challenge.
- Subjects
YELLOW fever; VIRAL vaccines; VIRUS diseases; PHYTOPLASMAS; MOUSE diseases; ALPHAVIRUSES
- Publication
Vaccines, 2020, Vol 8, Issue 3, p368
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines8030368