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- Title
Wnt5a attenuates Wnt/β-catenin signalling in human dermal papilla cells.
- Authors
Kwack, Mi Hee; Kim, Moon Kyu; Kim, Jung Chul; Sung, Young Kwan
- Abstract
Findings of recent studies have demonstrated modulation of Wnt/β-catenin signalling by Wnt5a, which is highly expressed in hair follicular dermal papilla ( DP) in vivo. Here, we investigated the question of whether Wnt5a can affect canonical Wnt/β-catenin signalling in DP cells. Treatment with Wnt5a resulted in attenuation of Wnt3a-mediated elevation of β-catenin signalling, which was increased by Wnt5a si RNA transfection in cultured DP cells, as examined by reporter assay. In addition, treatment with Wnt5a resulted in repressed Wnt3a-mediated expression of Axin2, EP2 and LEF1 in cultured DP cells, whereas Wnt5a si RNA transfection resulted in increased Wnt3a-mediated expression of the genes in isolated DPs of cultured hair follicles. Moreover, treatment with Wnt5a resulted in attenuation of Wnt3a-mediated accumulation of β-catenin in the nucleus in DP cells. Our data strongly suggest that Wnt5a acts as an autocrine factor and attenuates canonical Wnt signalling pathway in human DP cells.
- Subjects
WNT genes; SMALL interfering RNA; CATENINS
- Publication
Experimental Dermatology, 2013, Vol 22, Issue 3, p229
- ISSN
0906-6705
- Publication type
Article
- DOI
10.1111/exd.12101