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- Title
DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8<sup>+</sup> T cell responses.
- Authors
Jino Lee; Jihye Kim; Jeewon Lee; Sung Jae Shin; Eui-Cheol Shin
- Abstract
Purpose: T cell-mediated immune responses, and particularly activation of polyfunctional T cells that simultaneously produce multiple cytokines, are necessary for the control of Mycobacterium tuberculosis. In the present study, we examined if DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B (RpfB) elicits polyfunctional T cell responses in mice. Materials and Methods: C57BL/6 mice were immunized intramuscularly three times, at 3-week intervals, with RpfB-expressing plasmid DNA. For comparison, protein immunization was performed with recombinant RpfB in control mice. After immunization, RpfB-specific T cell responses were assessed by interferon-γ (IFN-γ) enzyme-linked immunosorbent spot assay and intracellular cytokine staining (ICS), and T cell polyfunctionality was assessed from the ICS data. Results: RpfB DNA immunization induced not only humoral immune responses, but also CD8+ and CD4+ T cell responses. Immunodominant T-cell epitopes were identified within RpfB by assays with overlapping peptides. RpfB DNA immunization elicited a polyfunctional CD8+ T cell response that was dominated by a functional phenotype of IFN-γ+/TNF-α+/IL-2-/CD107a+. Conclusion: RpfB DNA immunization elicits polyfunctional CD8+ T cell responses, suggesting that RpfB DNA immunization might induce protective immunity against tuberculosis.
- Subjects
IMMUNITY; IMMUNIZATION; GENES; TUBERCULOSIS; MYCOBACTERIAL diseases; ANTIVIRAL agents
- Publication
Clinical & Experimental Vaccine Research, 2014, Vol 3, Issue 2, p235
- ISSN
2287-3651
- Publication type
Article
- DOI
10.7774/cevr.2014.3.2.235